In vitro Antioxidant Activity of Methanolic Extract of Nelumbium speciosum

Authors

  • Anthoni Samy A Wallaga University
  • Geegi P.G Department of Biochemistry, St. Joseph’s College

DOI:

https://doi.org/10.20372/mhsr.v1i1.86

Keywords:

Nelumbium speciosum, Antioxidant activity, Free radicals

Abstract

The present study was conducted to evaluate the antioxidant activity of medicinal plant Nelumbium speciosum. The objectives of this study are to find the presence of secondary metabolites by preliminary phytochemical investigation and FTIR analysis in the N. speciosum methanolic leaf extract. This antioxidant activity study was tested for their free radical scavenging properties using ascorbic acid as standard antioxidant. Antioxidant activity of methanolic leaf extract of N. speciosum was determined by using different methods namely DPPH radical scavenging assay, ferric reducing assay, nitric oxide radical scavenging assay, superoxide radical scavenging assay and ABTS radical scavenging assay, and its IC 50 values were found to be 33.80, 662.20, 265.55, 219.5 and 20.34µg/ml. The extracts exhibited marked dose dependent in vitro antioxidant activity.

Downloads

Download data is not yet available.

Author Biographies

Anthoni Samy A, Wallaga University

School of Medicine, Wollega University, Post Box No: 395, Nekemte, Ethiopia

Geegi P.G, Department of Biochemistry, St. Joseph’s College

Department of Biochemistry, St. Joseph’s College, Trichy-620002, Tamil Nadu, India

References

Adwan, G., Abu-Shanab, B. and Adwan, K. (2010). Antibacterial activities of some plant extracts alone and in combination with different antimicrobials against multidrug–resistant Pseudomonas aeruginosa strains. Asian Pacific Journal of Tropical Medicine 3(4): 266-269.

Akinmoladun, A.C., Obuotor, E.M. and Farombi, E.O. (2010). Evaluation of antioxidant and free radical scavenging capacities of some Nigerian indigenous medicinal plants. Journal of Medicinal Food 13(2): 444-551.

Al-Dhabi, N.A., Balachandran, C., Raj, M.K., Duraipandiyan, V., Muthukumar, C., Ignacimuthu, S., Khan, I.A. and

Rajput, V.S. (2012). Antimicrobial, antimycobacterial and antibiofilm properties of Couroupita guianensis Aubl. fruit extract. BMC Complementary and Alternative Medicine 12: 242.

Ancy, P., Padmaja, V., Radha, K., Jose, J. and Hisham, A. (2013).Diuretic activity of the roots of Flacourtia indica. Hygeia: Journal for Drugs and Medicines 5(1): 79-83.

Apu, A., Muhit, M., Tareq, S., Pathan, A., Jamaluddin, A. and Ahmed, M. (2010). Antimicrobial activity and brine shrimp lethality bioassay of the leaves extract of Dillenia indica Linn. Journal of Young Pharmacists 2(1): 50–53.

Banu, S.K. and Cathrine, L. (2015). General techniques involved in phytochemical analysis. International Journal of Advanced Research in Chemical Science 2(4): 25-32.

Bhat, G.K. (2014). Flora of South Kanara. Akriti Prints, Mangalore, India.

Biswas, A. and Battu, G. (2016). Flacourtia indica (Burm. f.) prevents CCl4 induced rat liver damage by augmenting antioxidant enzyme activity. European Journal of Pharmaceutical and Medical Research 3(4): 263-270.

Chung, Y., Chien, C., Teng, K. and Chou, S. (2006). Antioxidative and mutagenic properties of Zanthoxylum ailanthoides Sieb & zucc. Food Chemistry 97: 418-425.

Cowan, M.M. (1999). Plant products as antimicrobial agents.

Clinical Microbiology Reviews 12(4): 564-582.

Deising, H.B., Reimann, S. and Pascholati, S.F. (2008). Mechanisms and significance of fungicide resistance. Brazilian Journal of Microbiology 39: 286-295.

Eramma, N. and Devaraja, G. (2013). Antibacterial potential and phytochemical analysis of Flacourtia indica (Burm.f.)

Merr. root extract against human pathogens. Indo American Journal of Pharm Research 3(5): 3832-3846.

Hasan, M.M., Chowdhury, S.P., Alam, S., Hossain, B. and Alam, M.S. (2005). Antifungal effects of plant extracts on seed-borne fungi of wheat seed regarding seed germination, seedling health and vigour index. Pakistan Journal of Biological Sciences 8(9): 1284-1289.

Hazra, B., Biswas, S. and Mandal, N. (2008). Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complementary and Alternative Medicine 8: 63.

Hsu, C. (2006). Antioxidant activity of extract from Polygonum aviculare L. Biol. Res. 39: 281-288.

Junaid, S., Rakesh, K.N., Dileep, N. and Kekuda, P.T.R. (2014). Antifungal, anthelmintic and insecticidal activity of ripe and unripe pericarp of Polyalthia longifolia (Annonaceae). Pharmanest 5(4): 2217-2220.

Karou, D., Dicko, M.H., Simpore, J. and Traore, A.S. (2005). Antioxidant and antibacterial activities of polyphenols from ethnomedicinal plants of Burkina Faso. African Journal of Biotechnology 4(8): 823-828.

Kavya, R., Shrungashree, R.M., Suchitra, S.V., Divakara, R. and Kekuda, P.T.R. (2010). Comparative study on antifungal activity and proximate composition of Abrus pulchellus Wall and Abrus precatorius Linn. Research Journal of Pharmacognosy and Phytochemistry 2(4): 284- 287.

Kekuda, P.T.R., Raghavendra, H.L., Solomon, T. and Duressa, D. (2016). Antifungal and antiradical potential of Moringa stenopetala (Baker f.) Cufod (Moringaceae). Journal of Bioscience and Agriculture Research 11(01): 923-929.

Koch, A., Kumar, N., Weber, L., Keller, H., Imani, J. and Kogel, K. (2013). Host-induced gene silencing of cytochrome P450 lanosterol C14α-demethylase–encoding genes confers strong resistance to Fusarium species. PNAS 110(48): 19324–19329.

Krishna, M.S. and Nair, J.A. (2010). Antibacterial, cytotoxic and antioxidant potential of different extracts from leaf, bark and wood of Tectona grandis. International Journal of Pharmaceutical Sciences and Drug Research 2(2): 155-158.

Kusirisin, W., Jaikang, C., Chaiyasut, C. and Narongchai, P. (2009). Effect of polyphenolic compounds from Solanum torvum on plasma lipid peroxidation, superoxide anion and cytochrome P450 2E1 in human liver microsomes. Medicinal Chemistry 5: 583-588.

Martins, N., Barros, L., Santos-Buelga, C., Silva, S., Henriques, M. and Ferreira, I.C.F.R. (2015). Decoction, infusion and hydroalcoholic extract of cultivated thyme: Antioxidant and antibacterial activities, and phenolic characterization. Food Chemistry 167: 131-137.

Maruthappan, V. and Shree, S.K. (2010). A report on the antioxidant activity of the powder of the entire plant of Phyllanthus reticulatus Poir. International Journal of Green Pharmacy 265-269.

McCartney, A.H., Foster, S.J., Fraaije, B.A. and Ward, E. (2003). Molecular diagnostics for fungal plant pathogens. Pest Management Science 59:129-142.

McLaughlin J.L. Methods in plant biochemistry. In Hostettmann K., (Ed). Assay for Bioactivity. Academic press, London, 1991: 1-32.

McLaughlin, J.L., Rogers, L.L. and Anderson, J.E. (1998). The use of biological assays to evaluate botanicals. Drug Information Journal 32: 513-524.

Molyneux, P. (2004). The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology 26(2): 211-219.

Murgananthan, G. and Pabbithi, S.C. (2012). Antimicrobial constituents from plants. International Research Journal of Pharmacy 3(1): 5-9.

Nair, R., Kalariya, T. and Chanda, S. (2005). Antibacterial activity of some selected Indian medicinal flora. Turkish Journal of Biology 29: 41-47.

Ndhlala, A.R., Chitindingu, K., Mupure, C., Murenje, T., Ndhlala, F., Benhura, M.A. and Muchuweti, M. (2008). Antioxidant properties of methanolic extracts from Diospyros mespiliformis (jackal berry), Flacourtia indica (Batoka plum), Uapaca kirkiana (wild loquat) and Ziziphus mauritiana (yellow berry) fruits. International Journal of Food Science and Technology 43(2): 284-288.

Okhale, S.E., Ugbabe, G.E., Bamidele, O., Ajoku, G.A. and Egharevba, H.O. (2014). Phytochemical and antimicrobial studies on extractives of Calyptrochilum emarginatum (SW) Schltr (Orchidaceae) growing in Nigeria. Journal of Medicinal Plants Research 8(4): 223-228.

Osman, H., Rahim, A.A., Isa, N.M. and Bakhir, N.M. (2009). Antioxidant activity and phenolic content of Paederia foetida and Syzygium aqueum. Molecules 14: 970-978.

Park, Y., Jung, S., Kang, S., Heo, B.G., Arancibia-Avila, P., Toledo, F., Drzewiecki, J., Namiesnik, J. and Gorinstein,

S. (2008). Antioxidants and proteins in ethylene-treated kiwifruits. Food Chemistry 107: 640-648.

Poornima, G., Manasa, M., Rudrappa, D. and Kekuda, P.T.R. (2012). Medicinal plants used by herbal healers in Narasipura and Manchale villages of Sagara Taluk, Karnataka, India. Science, Technology and Arts Research Journal 1(2): 12-17.

Raghavendra, H.L., Kekuda, P.T.R., Vijayananda, B.N., Duressa, D. and Solomon, T. (2016). Nutritive composition and antimicrobial activity of Moringa stenopetala (Baker f.) Cufod. Journal of Advances in Medical and Pharmaceutical Sciences 10(3): 1-9.

Rakesh, K.N., Junaid, S., Dileep, N., Vinayaka, K.S., Kekuda,

P.T.R. and Raghavendra, H.L. (2013). Antibacterial and antioxidant activity of Fahrenheitia zeylanica (Thw.) Airy. Science, Technology and Arts Research Journal 2(4):27- 33.

Ravikumar, A.R., Madgaonkar, V., Venkatesha, R.T., Bharathi, R. and Murthy, K.V. (2014). Potential cytotoxic drug effects of secondary metabolites derived from selected medicinal plants of Savanadurga forest in Karnataka. International Journal of Pharmacy and Pharmaceutical Sciences 6(10): 238-241.

Savithramma, N., Yugandhar, P., Devi, P.S., Ankanna, S., Suhrulatha, D., Prasad, K.S., Ranjani, R., Nagaraju, N. and Chetty, K.M. (2017). Documentation of ethnomedicinal information and antimicrobial validation of Thespesia populnea used by Yanadi tribe of Ganugapenta village, Chittoor district, Andhra Pradesh, India. Journal of Intercultural Ethnopharmacology 6(2): 158-169.

Shalaby, E.A. and Shanab, S.M.M. (2013). Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina

platensis. Indian Journal of Geo-Marine Sciences 42(5): 556-564.

Shukla, V., Vashistha, M. and Singh, S.N. (2009). Evaluation of antioxidant profile and activity of Amalaki (Emblica officinalis), Spirulina and wheat grass. Indian Journal of Clinical Biochemistry 24(1): 70-75.

Swapna, G., Jyothirmai, T., Lavanya, V., Swapnakumari, S. and Prasanna, S.L.A. (2015). Extraction, and characterization of bioactive compounds from plant extracts a review. European Journal of Pharmaceutical Science and Research 2(1): 1-6.

Thaipong, K., Boonprakob, U., Crosby, K., Cisneros-Zevallos,

L. and Byrne, D.H. (2006). Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis 19: 669-675.

Tiwari, P., Kumar, B., Kaur, M., Kaur, G. and Kaur, H. (2011).Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia 1(1): 98-106.

Tyagi, S., Singh, M., Singh, D., Yadav, I., Singh, S. and Mansoori, M.H. (2011). Anti-asthmatic potential of Flacourtia indica Merr. African Journal of Basic and Applied Sciences 3(5): 201-204.

Tyagi, S.N., Rakshit., Singh, A., Raghavendra., Saxena, A. and Patel, B.D. (2010). In vitro antioxidant activity of methanolic and aqueous extract of Flacourtia indica Merr. American-Eurasian Journal of Scientific Research 5(3): 201-206.

Varkey, J. and Thomas, J. (2011). Protective effect of Flacourtia indica (Burm.f) Merr. in methotrexate induced hepatotoxicity. Pharmanest 2(2-3): 115-123.

Wan, C., Yu, Y., Zhou, S., Liu, W., Tian, S. and Cao, S. (2011). Antioxidant activity and free radical-scavenging capacity of Gynura divaricata leaf extracts at different temperatures. Pharmacognosy Magazine 7(25): 40-45.

Wangcharoen, W. and Morasuk, W. (2007). Antioxidant capacity and phenolic content of holy basil. Songklanakarin Journal of Science and Technology 29(5): 1407-1415.

Wikaningtyas, P. and Sukandar, E.Y. (2016). The antibacterial activity of selected plants towards resistant bacteria isolated from clinical specimens. Asian Pacific Journal of Tropical Biomedicine 6(1): 16-19.

Yoon, M., Cha, B. and Kim, J. (2013). Recent trends in studies on botanical fungicides in agriculture. The Plant Pathology Journal 29(1): 1-9.

Downloads

Published

2023-04-29

How to Cite

Samy A, A., & P.G, G. (2023). In vitro Antioxidant Activity of Methanolic Extract of Nelumbium speciosum. Medical and Health Sciences Research Journal, 1(1), 70–75. https://doi.org/10.20372/mhsr.v1i1.86

Issue

Section

Articles

Plaudit