HPTLC Finger Printing Analysis of Aqueous Root Extract of Rotula aquatica Lour. for Steroid and Terpenoid Contents
DOI:
https://doi.org/10.20372/mhsr.v1i1.84Keywords:
Rotula aquatica, HPTLC, Phytochemical, Steroids, TerpenoidsAbstract
The medicinal plants are considered to be the most important in the healing system due to their qualities. There is a high demand for the medicinal plants in pharmaceutical and cosmetic industry. The medicinal plants acquire these qualities due to the phyotchemicals present in them. The plant products are friendly to the user and poses minimum or no side effects hence they are preferred for treatment. The plants have many secondary metabolites that give them protection from different biotic and abiotic stresses. The objective of present work was to study the HPTLC fingerprinting of aqueous root extract of Rotula aquatica. A CAMAG HPTLC system equipped with LINOMAT 5 applicator, a TLC scanner3, REPROSTAR with 12 bit CCD camera and WIN CATS-4 software were used. In the present study aqueous extract of Rotula aquatica plant roots contains steroids and terpenoids in addition to different other components. The HPTLC fingerprinting profile developed for aqueous extract of Rotula aquatic will help in proper identification of compounds. By isolating and identifying marker compounds, new drugs can be formulated to treat various ailments.
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