Radical Scavenging, Cytotoxic and Antimicrobial Activity Flacourtia indica (Burm. f.) Merr.
DOI:
https://doi.org/10.20372/mhsr.v1i1.87Keywords:
Flacourtia indica, Brine shrimp,, Agar well diffusion, Poisoned food technique, DPPHAbstract
In the present study, we determined antiradical, cytotoxic and antimicrobial potential of leaf extract of Flacourtia indica (Burm. f.) Merr. (Salicaceae). Antiradical activity of extract was evaluated by DPPH and ABTS assay. Cytotoxic activity was screened by brine shrimp lethality assay. Antibacterial and antifungal activity of extract was assessed by Agar well diffusion and Poisoned food technique respectively. Leaf extract was found to scavenge DPPH and ABTS radicals dose dependently with an IC 50 value of 20.41 and 1.20µg/ml respectively. The extract was shown to exhibit concentration dependent mortality of brine shrimp larvae with an IC 50 value of 283.27µg/ml. Extract exhibited inhibitory activity against all test bacteria with highest and least activity against B. subtilis and E. coli respectively. The extract has shown varied antifungal activity against 6 seed-borne mycoflora. Curvularia sp. and Aspergillus fumigatus exhibited highest and least susceptibility to leaf extract. From the results of this study, it is found that the plant is a good resource that can be used for developing antiradical, cytotoxic and antimicrobial agents.
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References
Anon (1977). Chinese Materia Medica, Jiangsu New Medical College, ed. Shanghai: Shanghai People’s Pub. House, 1810.
Arnao, M.B., Cano, A., Acosta, M. (2001). The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chemistry 73: 239-244.
Buhler, D.R., Miranda, C. (2000). Antioxidant activities of flavonoids. The Linus Pauling Institute.
Gooijer, C., Evade Rijke., Zappey, H. (1997). Liquid chromatography with atmospheric pressure chemical ionization and electrospray ionization mass spectrometry of flavonoids with triplequadrupole and ion-trap instruments. Journal of Chromatography 984: 45-48.
Idu, M., Omogbai, E. K. I., Aghimine, G. E. Amaechina, F.,Timothy, O. & Omonigho, S. E. (2007). Preliminary phytochemistry, antimicrobial properties and acute toxicity of Stachytarpheta jamaicensis (L.) Vahl. leaves, Trends in Medical Research 2:193-198.
Kareru, P.G., Keriko, Gachanja, J.M., Kenji, A.N. (2008). Direct detection of triterpenoid saponins in medicinal plants. African Journal of Traditional, Complementary and Alternative Medicines 5(1): 56 60.
Kekuda, P.T.R., Manasa, M., Poornima, G., Abhipsa, V., Rekha, C., Shivaleela, P Upashe., Raghavendra, H.L. (2013). Antibacterial, Cytotoxic and Antioxidant Potential of Vitex Negundo Var. Negundo and Vitex Negundo Var. Purpurascens –A Comparative Study. Science Technology and Arts Research Journal 2(3): 59-68.
Khushad, M.M., Masiunas, J., Smith, M.A.L., Kalt, W., Eastmank. (2003). Health promoting phytochemicals in vegetable. Horticultural Reviews 28: 125-185.
Kinsella, J.E., Frankel E., Chao, W.W. (1993). Possible mechanism for the protective role of the antioxidant in wine and plant foods. Food Technology 47: 85-89.
Kokate, C.K. (1999). Practical Pharmacognosy. Vallabh Prakashan, New Delhi 149-156.
Koleva, I.I., Van Beek, T.A., Linssen, J.P.H., de G root,, A., Evstatieva L.N (2002). Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis 13: 8-17.
Lai, L.S., Chou, S.T., German, B., Kanner, J. (2001). Studies on the antioxidative activities of Hsiantsao (Mesonaprocumbens Hemsl.) leaf gum. Journal of Agricultural and Food Chemistry 49: 936-968.
Luo X (2005). Simultaneous analysis of N-nornuciferine, O- nornuciferine, nuciferine, and roemerine in leaves of Nelumbo nucifera Gaertn by high-performance liquid chromatography– photodiode array detection-electrospray mass spectrometry. Analytica Chimica Acta 538: 129-133.
Marcocci, L., Maguire, J.J., Droy-Lefaix, M.T., Packer, L. (1994). The nitric oxide scavenging properties of Ginkgobiloba extract. Biochemical and Biophysical Research Communications 201: 748-755.
Muruganantham, S., Anbalagan, G., Ramamurthy, N. (2009). FT-IR and SEM-EDS Comparative Analysis of Medicinal Plants, Eclipta Alba Hassk and Eclipta prostrata Linn. Romanian Journal of Biophysics 19(4): 285 294.
Nikhat, F., Satynarayana, D., Subhramanyam, E.V.S. (2009). Isolation, Characterization and Screening of Antioxidant Activity of the Roots of Syzygiumcuminii (L) Skeel. Asian Journal of Research in Chemistry 218-221.
Nishimiki, M., Rao, N.A., Yagi, K. (1972). The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochemical and Biophysical Research Communications 46: 849-853.
Peryor, W.A. (1991). The antioxidant nutrient and disease prevention-what do we know and what do we need to find out? American Journal of Clinical Nutrition 53: 391-393.
Pietta, P., Simonetti, P., Mauri, P. (1998). Antioxidant activity of selected medicinal plants. Journal of Agricultural and Food Chemistry 46: 4487-90.
Rice-Evans, C.A., Sampson, J., Bramley, P.M, (1997). Why do we expect carotenoids to be antioxidants in vivo? Free Radical Research 26:381–98
Shahidi, F., Wanasundara, P.D. (1992). Phenolic antioxidants. Critical Reviews in Food Science and Nutrition 32: 67- 103.
Singleton, V.L., Rossi, J.A., (1965). Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. American Journal of Enology and Viticulture 16: 144-158.
Sridhar KR, Bhat R (2007). Lotus: a potential nutraceutical source. Journal of Agricultural Technology 3: 143–155.
Vinson, J. A., Su, X., Zubik, L., and Bose, P. (2001). Phenol antioxidant quantity and quality in foods: fruits. Journal of Agricultural and Food Chemistry, 49(11): 5315-5321.
Wang, Q.C. and Zhang, X.Y. (2004). Lotus flower cultivars in China. China Forestry Publ. House. Beijing.
Yen, G.C., Chen, H.Y. (1995). Antioxidant activity of various tea extracts in relation to their antimutagenicity. Journal of Agricultural and Food Chemistry 43: 27-32.
Yildirim, A., Mavi, A., Kara, A. A. (2001). Determination of antioxidant and antimicrobial activities of Rumex crispus
L. extracts. Journal of Agricultural and Food Chemistry 9: 4083-4089.
Zhishen, J., Mengcheng, T., Janming, W. (1999). The determination of flavonoids contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64: 555-559.
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