Antibacterial Activity of Three Parmotrema Species from Western Ghats of Karnataka against Clinical Isolates of Burn and Dental Caries

Authors

  • Vivek MN Sahyadri Science College
  • Yashoda Kambar Sahyadri Science College
  • Manasa M Sahyadri Science College
  • Prashith Kekuda TR Sahyadri Science College

Keywords:

Western Ghats, Parmotrema, Antibacterial activity, Burn, Dental caries

Abstract

The present study was conducted to evaluate the antibacterial efficacy of methanol extract of three species of macrolichen Parmotrema (Parmeliaceae) viz., P.tinctorum (Nyl.) Hale, P. grayanum (Hue.) Hale and P. praesorediosum (Nyl.) Hale from Western Ghats of Karnataka against clinical isolates of burn and dental caries. Identification of lichens was done by morphological, anatomical and color tests. The powdered lichen materials were extracted using methanol in soxhlet apparatus. Inhibitory potential of lichen extracts was evaluated against two isolates of Staphyloccus aureus (Sa-01 and Sa-02 from burn subjects) and two isolates of Streptococcus mutans (Sm-01 and Sm-02 from dental caries) by agar well diffusion assay. Lichen extracts were found inhibitory against all clinical isolates and the inhibitory activity was dose dependent. Among S. aureus and S. mutans isolates, Sa-02 and Sm-02 were inhibited to higher extent respectively by lichen extracts. Extract of P. praesorediosum inhibited S. aureus isolates to higher extent. Extracts of P. praesorediosum and P. tinctorum inhibited S. mutans isolates to higher extent. The lichens of this study were found promising sources for development of agents active against clinical isolates. The observed activity of extracts could be ascribed to the presence of secondary metabolites.

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Author Biographies

Vivek MN, Sahyadri Science College

P.G. Department of Studies and Research in Microbiology, Sahyadri Science College (Autonomous), Kuvempu University, Shivamogga-577203, Karnataka, India

Yashoda Kambar, Sahyadri Science College

P.G. Department of Studies and Research in Microbiology, Sahyadri Science College (Autonomous), Kuvempu University, Shivamogga-577203, Karnataka, India

Manasa M , Sahyadri Science College

P.G. Department of Studies and Research in Microbiology, Sahyadri Science College (Autonomous), Kuvempu University, Shivamogga-577203, Karnataka, India

Prashith Kekuda TR, Sahyadri Science College

P.G. Department of Studies and Research in Microbiology, Sahyadri Science College (Autonomous), Kuvempu University, Shivamogga-577203, Karnataka, India

References

Awasthi, D.D. (2000). A compendium of the macrolichens from India, Nepal and Sri Lanka. Bishen Singh Mahendra Pal Singh, Dehra Dun, India.

Balaji, P., Hariharan, G.N. (2007). In vitro antimicrobial activity of Parmotremapraesorediosum thallus extracts. Research Journal of Botany 2(1): 54-59.

Benatti, M.N., Gernert, M., Schmitt, I. (2013). Parmotrema hydrium, a new species of Parmeliaceae in southeastern Brazil. Acta Botanica Brasilica 27(4): 810-814.

Chauhan, R., Abraham, J. (2013). Invitro antimicrobial potential of the lichen Parmotrema sp. extracts against various pathogens. Iranian Journal of Basic Medical Sciences16(7):882-885.

Culberson, C.F., Kristinsson, H. (1970). A standardized method for the identification of lichen products. Journal of Chromatography 46: 85-93.

Culberson, C.F. (1972). Improved conditions and new data for the identification of lichen products by a standardized thin layer chromatographic method. Journal of Chromatography 72: 113-125.

Davies, J., Davies, D. (2010). Origins and evolutions of antibiotic resistance. Microbiology and Molecular Biology Reviews 74(3): 417-433.

Divakar, P.K., Upreti, D.K. (2005). Parmelioid lichens in India. Bishen Singh and Mahendra Pal Singh, Dehra Dun, India.

Giedraitiene, A., Vitkauskiene, A., Naginiene, R., Pavilonis, S. (2011). Antibiotic resistance mechanisms of clinically important bacteria.Medicina (Kaunas) 47(3): 137-146.

Ingolfsdottir, K., Hjalmarsdottir, H.A., Sigurdsson, A., Gudjonsdottir, G.A., Brynjolfsdottir, A., Steingrimsson, O. (1997). In vitro susceptibility of Helicobacterpylori to protolichesterinic acid from the lichen Cetraria islandica. Antimicrobial agents and Chemotherapy 41(1): 215-217.

Javeria, S., Shahi, S.K., Shahi, M.P., Upreti, D.K. (2013). Parmotrema nilgherrense: potential antimicrobial activity against drug resistant pathogens. International Journal of Microbial Resource Technology 2(1): 36-40.

Jayalal, U., Divakar, P.K., Joshi, S., Oh, S., Koh, Y., Hur, J. (2013). The lichen genus Parmotrema in South Korea. Mycobiology 41(1): 25-36.

Kekuda, P.T.R., Junaid, S., Dileep, N., Rakesh, K.N., Vinayaka, K.S. (2013). Anticaries activity of Usnea pictoides G. Awasthi-A macrolichen from Western Ghats of Karnataka, India. Science Technology and Arts Research Journal 2(4): 87-90.

Kekuda, P.T.R., Raghavendra, H.L., Swathi, D., Venugopal, T.M., Vinayaka, K.S. (2012). Antifungal and cytotoxic activity of Everniastrum cirrhatum (Fr.) Hale. Chiang Mai Journal of Science 39(1): 76-83.

Kekuda, P.T.R., Vinayaka, K.S., Swathi, D., Suchitha, Y., Venugopal, T.M., Mallikarjun, N. (2011). Mineral composition, total phenol content and antioxidant activity of a macrolichen Everniastrum cirrhatum (Fr.) Hale (Parmeliaceae). E-Journal of Chemistry 8(4): 1886-1894.

Kumar, A.H.S., Kekuda, P.T.R., Vinayaka, K.S., Swathi, D., Venugopal, T.M. (2011). Anti-obesity (Pancreatic lipase inhibitory) activity of Everniastrum cirrhatum (Fr.) Hale (Parmeliaceae). Pharmacognosy Journal 3(19): 65-68.

Kumar, S.V.P., Kekuda, P.T.R., Vinayaka, K.S., Sudharshan, S.J., Mallikarjun, N., Swathi, D. (2010). Studies on antibacterial, anthelmintic and antioxidant activities of a macrolichen Parmotremapseudotinctorum (des. Abb.) Hale (Parmeliaceae) from Bhadra wildlife sanctuary, Karnataka. International Journal of PharmTech Research 2(2): 1207-1214.

Oh, S., Jeon, H., Lim, K., Koh, Y., Hur, J. (2006). Antifungal activity of lichen-forming fungi isolated from Korean and Chinese lichen species against plant pathogenic fungi. Plant Pathology Journal 22(4): 381-385.

Pavithra, G.M., Vinayaka, K.S., Rakesh, K.N., Junaid, S., Dileep, N., Kekuda, P.T.R., Siddiqua, S., Naik, A.S. (2013). Antimicrobial and antioxidant activities of a macrolichen Usnea pictoides G. Awasthi (Parmeliaceae). Journal of Applied Pharmaceutical Science3(8): 154-160.

Sinha, S.N., Biswas, M. (2011). Evaluation of antibacterial activity of some lichen from Ravangla, Sikkim, India. International Journal of Pharma and Bio Sciences 2(4): B23-B28.

Smith, R.D., Coast, J. (2002). Antimicrobial resistance: a global response. Bulletin of the World Health Organization 80(2): 126-133.

Thadhani, V.M., Choudhary, I.M., Khan, S., Karunaratne, V. (2012). Antimicrobial and toxicological activities of some depsides and depsidones. Journal of the National Science Foundation Sri Lanka 40(1):43-48.

Turk, A.O., Yılmaz, M., Kivan, M., Turk, H.(2003). The antimicrobial activity of extracts of the lichen Cetraria aculeata and its protolichesterinic acid constituent. Zeitschrift fur Naturforschung 58c: 850-854.

Turk, H., Yilmaz, M., Tay, T., Turk, A.O., Kivanc, M. (2006). Antimicrobial activity of extracts of chemical races of the lichen Pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents. Zeitschrift fur Naturforschung61c: 499-507.

Verma, N., Behera, B.C., Parizadeh, H., Sharma, B.O. (2011). Bactericidal activity of some lichen secondary compounds of Cladonia ochrochlora, Parmotrema nilgherrensis and Parmotremasancti-angelii. International Journal of Drug Development & Research 3(3): 222-232.

Vinayaka, K.S., Krishnamurthy, Y.L., Kekuda, P.T.R., Kumar,P.S.V., Sudharshan, S.J., Chinmaya, A. (2009). Larvicidal and wormicidal efficacy of methanolic extracts of five macrolichens collected from Bhadra wildlife sanctuary. Biomedicine 29(4): 327-331.

Vinayaka, K.S., Krishnamurthy, Y.L. (2012). Ethno-licheno- logical studies of Shimoga & Mysore districts, Karnataka, India. Advances in Plant Sciences 25(1): 265-267.

Vinayaka, K.S., Shetty, S., Krishnamurthy, Y.L. (2011). Utilization of lichens in the central Western Ghats area of Karnataka. British Lichenological Society Bulletin 109: 56- 62.

Vivek, M.N., Manasa, M., Pallavi, S., Swamy, S.H.C., Kekuda, P.T.R. (2013). Antibacterial potential of cashew apple (Anacardium occidentale L.) juice against clinical isolates of Staphylococcus aureus and Streptococcus mutans. Science Technology and Arts Research Journal 2(3): 144-146.

Yilmaz, M., Turk, A.O., Tay, T., Kivanc, M. (2004). The antimicrobial activity of extracts of the lichen Cladonia foliacea & its (-)-usnic acid, atranorin & fumarprotocetraric acid constituents. Zeitschrift fur Naturforschung 59c: 249- 254.

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Published

28.03.2014

How to Cite

MN, V., Kambar, Y., M , M., & TR, P. K. (2014). Antibacterial Activity of Three Parmotrema Species from Western Ghats of Karnataka against Clinical Isolates of Burn and Dental Caries. Journal of Science, Technology and Arts Research, 3(1), 132–135. Retrieved from https://journals.wgu.edu.et/index.php/star/article/view/511

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