Inhibitory Activity of Polyalthia longifolia, Anaphalis lawii and Gnidia glauca against Colletotrichum capsici and Urinary Tract Pathogens
Keywords:
Polyalthia longifolia, Anaphalis lawii, Gnidia glauca, Colletotrichum capsici, Poisoned food technique, Urinary tract infections, Agar well diffusionAbstract
The present study was conducted with an aim of determining inhibitory effect of extracts from different parts of Polyalthia longifolia (leaf, ripe and unripe pericarp), Anaphalis lawii (leaf and flower), Gnidia glauca (leaf, bark and flower) against Colletotrichum capsici and urinary tract pathogens. The shade dried plant materials were extracted using methanol. Antifungal effect of extracts was evaluated against Colletotrichum capsici isolated from anthracnose of chilli by Poisoned food technique. Antibacterial activity of extracts was determined against five antibiotic resistant bacteria isolated from urinary tract infections by Agar well diffusion assay. The extracts caused marked inhibition of mycelial growth of C. capsici as indicated by reduced diameter of fungal colonies on poisoned plates. Extracts of P. longifolia inhibited C. capsici to higher extent followed by A. lawii and G. glauca. All extracts from selected plants inhibited test bacteria but to varied extent. Susceptibility was higher in case of Gram positive when compared to Gram negative bacteria. Staphylococcus aureus and Escherichia coli were inhibited to higher extent among Gram positive and Gram negative bacteria respectively. Among plants, P. longifolia caused higher inhibition of test bacteria. The extracts from the selected plants can be considered as promising sources of bioactive agents which can be used to treat urinary tract infections caused by antibiotic resistant bacteria and to control anthracnose of chilli. Further studies are to be carried in order to isolate and characterize bioactive principles from extracts and to determine their inhibitory potential against C. capsici and uropathogens.
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Amin, M., Mehdinejad, M., Pourdangchi, Z. (2009). Study of bacteria isolated from urinary tract infections and determination of their susceptibility to antibiotics. Jundishapur Journal of Microbiology 2(3): 118-123.
Anand, T., Raguchander, T., Karthikeyan, G., Gopala krishnan, C., Bhaskaran, R., Ganeshamoorthi, P. (2007). Induction of systemic resistance by plant grown promoting rhizobacteria in chilli plants against fruit rot disease. World Journal of Agricultural Sciences 3(6): 815-824.
Beyene, G., Tsegaye, W. (2011). Bacterial uropathogens in urinary tract infection and antibiotic susceptibility pattern in Jimma university specialized hospital, Ethiopia. Ethiopian Journal of Health Sciences 21(2): 141-146.
Carmeli, Y., Troillet, N., Eliopoulos, G.M., Samore, M.H. (1999). Emergence of antibiotic resistant Pseudomonas aeruginosa: Comparison of risks associated with different antipseudomonal agents. Antimicrobial Agents and Chemotherapy 43(6): 1379-1382.
Chutia, M., Bhuyan, D.P., Pathak, M.G., Sarma, T.C., Boruah, P. (2009).Antifungal activity and chemical composition of Citrus reticulata Blanco essential oil against phytopathogens from North East India. LWT-Food Science and Technology 42: 777-780.
Cooke, M.D. (1976). Antibiotic resistance among coliform and fecal coliform bacteria isolated from sewage, seawater, and marine shellfish. Antimicrobial Agents and Chemotherapy 9(6): 879-884.
Davies, J., Davies, D. (2010).Origins and evolutions of antibiotic resistance. Microbiology and Molecular Biology Reviews 74(3): 417-433.
Demain, A.L., Sanchez, S. (2009). Microbial drug discovery: 80 years of progress. Journal of Antibiotics 62: 5-16.
Dileep, N., Junaid, S., Rakesh, K.N., Kekuda, P.T.R., Nawaz,
A.S.N. (2013).Antifungal activity of leaf and pericarp extract of Polyalthia longifolia against pathogens causing rhizome rot of ginger. Science, Technology and Arts Research Journal 2(1): 56-59.
Farooq, M.A., Iqbal, U., Iqbal, S.M., Afzal, R., Rasool, A. (2010). In-vitro evaluation of different plant extracts on mycelial growth of Sclerotium rolfsii the cause of root rot of sugar beet. Mycopath 8(2): 81-84.
Fathima, S.K., Bhat, S.S., Girish, K. (2009). Efficacy of some essential oils against Phomopsis azadirachtae- the incitant of die-back of neem. Journal of Biopesticides 2(2): 157-160.
Humayun, T., Iqbal, A. (2012).The culture and sensitivity pattern of urinary tract infections in females of reproductive age group. Annals of Pakistan Institute of Medical Sciences 8(1): 19-22.
Kambar, Y., Vivek, M.N., Manasa, M., Kekuda, P.T.R., Nawaz, N.A.S. (2013). Inhibitory effect of cow urine against Colletotrichum capsici isolated from anthracnose of chilli (Capsicum annuum L.). Science, Technology and Arts Research Journal 2(4): 91-93.
Kannan, R.R., Arumugam, R., Anantharaman, P. (2012).Chemical composition and antibacterial activity of Indian seagrasses against urinary tract pathogens. Food Chemistry 135(4): 2470-2473.
Kekuda,P.T.R., Raghavendra, H.L., Surabhi, K.S., Preethi, H.R.,Swarnalatha, S.P. (2010). Cytotoxic activity of methanol extract of Abrus pulchellus Wall (Fabaceae) leaves. Biomedicine 30(3): 377-379.
Kyabaggu, D., Ejobi, F., Olila, D. (2007).The sensitivities to first-line antibiotic therapy of the common urinary tract bacterial infections detected in urine samples at a hospital in metropolitan Kampala (Uganda). African Health Sciences 7(4): 214-222.
Lodhia, M.H., Bhatt, K.R., Thaker, V.S. (2009). Antibacterial activity of essential oils from Palmarosa, Evening Primrose, Lavender and Tuberose. Indian Journal of Pharmaceutical Sciences 71(2): 134-136.
Manasa, M., Kambar, Y., Swamy, S.H.C., Vivek, M.N., Kumar, R.T.N., Kekuda, P.T.R. (2013). Antibacterial efficacy of Pimenta dioica (Linn.) Merill and Anacardium occidentale L. against drug resistant urinary tract pathogens. Journal of Applied Pharmaceutical Science 3(12): 72-74.
Masoodi, L., Anwar, A., Ahmed, S., Sofi, T.A. (2013). Cultural, morphological and pathogenic variability in Colletotrichum capsici causing die-back and fruit rot in chilli. Asian Journal of Plant Pathology 7(1): 29-41.
Mohammadi, P., Lotfi, N., Naseri, L., Etebarian, H.R. (2013). Antifungal activities of essential oils from some Iranian medicinal plants against various post harvest moulds. Journal of Medicinal Plant Research 7(23): 1699-1708.
Nalubega, R., Kabasa, J.D., Olila, D., Kateregga, J. (2011). Evaluation of antibacterial activity of selected ethnomedicinal plants for poultry in Masaka District, Uganda. Research Journal of Pharmacology 5(2): 18-21.
Narasimhan, A., Shivakumar, S. (2012). Study of mycolytic enzymes of Bacillus sp. against Colletotrichum gloeosporioides causing anthracnose in Chilli. Acta Biologica Indica 1(1): 81-89.
Oerke, E.C. (2006). Crop losses to pests. Journal of Agricultural Science 144: 31-43.
Okonko, I.O., Ijandipe, L.A., Ilusanya, A.O., Donbraye- Emmanuel,O.B., Ejembi, J., Udeze, A.O., Egun, O.C., Fowotade, A., Nkang, A.O. (2010). Detection of Urinary Tract Infection (UTI) among pregnant women in Oluyoro Catholic Hospital, Ibadan, South-Western Nigeria. Malaysian Journal of Microbiology 6(1): 16-24.
Onanuga, A., Awhowho, G.O. (2012). Antimicrobial resistance of Staphylococcus aureus strains from patients with urinary tract infections in Yenagoa, Nigeria. Journal of Pharmacy and Bioallied Sciences 4(3): 226-230.
Peneira, R.S., Sumitha, T.C., Furlan, M.R., Jorge, A.O., Ueno, M. (2004). Antibacterial activity of essential oils on microorganisms isolated from urinary tract infection. Revista de Saude Publica 38(2): 326-328.
Rakesh, K.N., Dileep, N., Junaid, S., Kekuda, P.T.R., Vinayaka, K.S., Nawaz, N.A.S. (2013). Inhibitory effect of cow urine extracts of selected plants against pathogens causing rhizome rot of ginger. Science, Technology and Arts Research Journal 2(2): 92-96.
Ratanacherdchai, K., Wang, H.K., Lin, F.C., Soytong, K. (2007). RAPD analysis of Colletotrichum species causing chilli anthracnose disease in Thailand. Journal of Agricultural Technology 3(2): 211-219.
Sahoo, S., Panda, P.K., Mishra, S.R., Parida, R.K., Ellaiah, P., Dash, S.K. (2008). Antibacterial activity of Barringtonia acutangula against selected urinary tract pathogens. Indian Journal of Pharmaceutical Sciences 70(5): 677-79.
Shifali, I., Gupta, U., Mohmood, S.E., Ahmed, J. (2012). Antibiotic susceptibility patterns of urinary pathogens in female outpatients. North American Journal of Medical Sciences 4(4): 163-169.
Than, P.P., Prihastuti, H., Phoulivong, S., Taylor, P.W.J., Hyde, K.D. (2008).Chilli anthracnose disease caused by Colletotrichum species. Journal of Zhejiang University Science B 9(10): 764-778.
Ushakiran, L., Chhetry, G.K.N., Singh, N.I. (2006). Fruit rot diseases of chilli and their management in agro-climatic conditions of Manipur. Journal of Mycopathological Research 44(2): 257-262.
Vivek, M.N., Kambar, Y, Pallavi, S, Kekuda, P.T.R., Kumar,
R.T.N. (2013b). Inhibitory effect of Anisomeles indica Linn. against multidrug resistant urinary tract pathogens. International Journal of Current Microbiology and Applied Sciences 2(12): 411-417.
Vivek, M.N., Kambar, Y., Manasa, M., Pallavi, S., Kekuda,P.T.R. (2013a). Bio control potential of Pimenta dioica and Anacardium occidentale against Fusarium oxysporum f.sp. zingiberi. Journal of Biological and Scientific Opinion 1(3): 193-195.
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