Isolation and Molecular Methods for the Identification of Fusarium solani from Solid Waste

Abstract

A major goal in microbial ecology is to link specific microbial populations to environmental processes. Solid waste degradation is an imperative aspect of environmental processes mediated by microorganisms individually or symbiotically. In the current study an attempt has been made to isolate and identify a microorganism which can degrade the hazardous xenobiotic compound propaxur commonly known as baygon from solid waste by both in vitro and in silico approaches. 28s rDNA gene has indicated the organism as Fusarium solani. Further studies were extended through bioinformatics approaches like BLAST (Basic Local Alignment Search Tool) and MSA (Multiple Sequence Analysis) to determine the relative phylogeny of this organism. This approach has shed light on evolutionary relationship among the organisms taken in the study. By this approach we have identified the Fusarium solani.