Isolation of Diesel, Engine Oil and Crude Oil Degrading Fungi from Oil Contaminated Soil for their Bioremediation Potential
DOI:
https://doi.org/10.20372/afnr.v1i1.71Keywords:
Biodegradation, PAHs, Fungi, Crude oilAbstract
Polycyclic aromatic hydrocarbons (PAHs) are considered to be most significant environmental pollutants and are to be removed. PAHs exposure causes damage to lungs, liver, kidneys, intestines and other internal organs and many PAHs are reported as carcinogenic, teratogenic, immuno-toxic and genotoxic. In the present study, the biodegradation of used engine oil, diesel oil and crude oil (as model PAHs) using fungal isolates was carried out. Total of 20 indigenous fungal isolate has been successfully isolated from oil contaminated soil. All the pure fungal isolates were checked for their PAHs utilization capability. Among the isolates tested 2 fungi namely, OK-6 and CSN-01 isolates showed potent crude oil, diesel and engine oil degradation ability with 52.45 %, 63.50 % and 48 % and 58.21 %, 69.64 % and 43.6 % respectively. The preliminary screening tests were conducted in MSM agar plates for diesel and engine oil degradation studies, followed by DCPIP method. Further characterization of the isolates, biosurfactant and enzyme properties are studied to optimize and enhance the bioremediation process.
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