Antimicrobial Activity of Selected Corticolous Macrolichens

Authors

  • Prashith Kekuda T.R SRNMN College of Applied Sciences
  • Ranjitha M.C SRNMN College of Applied Sciences
  • Ghazala Firdose SRNMN College of Applied Sciences
  • Vidya P SRNMN College of Applied Sciences
  • Vinayaka K.S Kumadvathi First Grade College

Keywords:

Lichens, Antimicrobial, Agar well diffusion, Poisoned food technique

Abstract

The present study was conducted to screen antimicrobial activity of three foliose macrolichens viz., Parmotrema reticulatum (Taylor) M. Choisy (Parmeliaceae), Heterodermia obscurata (Nyl.) Trevis (Physciaceae) and Dirinaria consimilis (Stirt.) D.D. Awasthi (Physciaceae) and two fruticose macrolichens viz., Ramalina pacifica Asahina (Ramalinaceae), and Ramalina hossei H. Magn & G. Awasthi (Ramalinaceae) growing on barks of areca trees. The lichens were identified on the basis of morphological, anatomical and color tests and the secondary metabolites (lichen substances) were identified by thin layer chromatography. Antibacterial activity of lichen extracts was evaluated against three Gram positive and five Gram negative bacteria by Agar well diffusion assay. The lichen extracts were effective in inhibiting all test bacteria. Overall, extract of D. consimilis and P. reticulatum displayed marked and least antibacterial efficacy. Marked inhibitory activity was observed against Klebsiella pneumoniae. Antifungal effect of lichen extracts was determined against four molds by Poisoned food technique. Among fungi, the growth of Colletrotrichum capsici was suppressed to higher extent by lichen extracts. The observed inhibitory activity of lichens could be ascribed to the presence of lichen substances.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biographies

Prashith Kekuda T.R, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S campus, Balraj Urs Road,
Shivamogga-577201, Karnataka, India

Department of Microbiology, Sahyadri Science College (Autonomous), Shivamogga-577203, 577203, Karnataka , India

Ranjitha M.C, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S campus, Balraj Urs Road,
Shivamogga-577201, Karnataka, India

Ghazala Firdose, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S campus, Balraj Urs Road,
Shivamogga-577201, Karnataka, India

Vidya P, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S campus, Balraj Urs Road,
Shivamogga-577201, Karnataka, India

Vinayaka K.S, Kumadvathi First Grade College

Department of Botany, Kumadvathi First Grade College, Shikaripura, Karnataka

References

Anjali, D.B., Mohabe, S., Reddy, A.M., Nayaka, S. (2015). Antimicrobial activities of 2-Propanol crude extract from lichen Parmotrema tinctorum (Despr.ex. Nyl.) Hale, collected from Eastern Ghats, India. Current Research in Environmental and Applied Mycology 5(3): 160-168.

Awasthi, D.D. (2000). A compendium of the macrolichens from India, Nepal and Sri Lanka. Bishen Singh Mahendra Pal Singh, Dehradun, India.

Babiah, P.S., Upreti, D.K., John, S.A. (2015). Assessment of fungicidal potential of lichen Heterodermia leucomelos (L.) Poelt against pathogenic fungi. Current Research in Environmental & Applied Mycology 5 (2): 92-100.

Balaji, P., Hariharan, G.N. (2013). Diversity of macrolichens in Bolampatti II forest range (Siruvani hills), Western Ghats, Tamil Nadu, India. ISRN Biodiversity 2013: 1-7, Article ID 124020. http://dx.doi.org/10.1155/2013/124020.

Chait, R., Vetsigian, K., Kishony, R. (2012). What counters antibiotic resistance in nature? Nature Chemical Biology 8: 2-5.

Chaves, T.P., Clementino, E.L.C., Felismino, D.C., Alves, R.R.N., Vasconcellos, A., Coutinho, H.D.M., Medeiros,

A.C.D. (2015). Antibiotic resistance modulation by natural products obtained from Nasutitermes corniger (Motschulsky, 1855) and its nest. Saudi Journal of Biological Sciences 22(4): 404-408.

Culberson, C.F. (1972). Improved conditions and new data for the identification of lichen products by a standardized

thin layer chromatographic method. Journal of Chromatography 72: 113-125.

Culberson, C.F., Kristinsson, H. (1970). A standardized method for the identification of lichen products. Journal of Chromatography 46: 85-93.

Devi, A.B., Mohabe, S., Reddy, M.A., Nayaka, S. (2015). Efficacy of a potential lichen Parmotrema andinum (Müll. Arg.) Hale against pathogenic microorganisms. Journal on New Biological Reports 4(2): 149-156.

Dudani, S.N., Nayaka, S., Mahesh, M.K., Chandran, S.M.D., Ramachandra, T.V. (2015). Lichen diversity in the sacred forest fragments of central Western Ghats. Journal of Biodiversity Management and Forestry 4:2.

Ghate, N.B., Chaudhuri, D., Sarkar, R., Sajem, A.L., Panja, S., Rout, J., Mandal, N. (2013). An antioxidant extract of tropical lichen, Parmotrema reticulatum, induces cell cycle arrest and apoptosis in breast carcinoma cell line MCF-7. PLoS One 2013; 8(12): e82293.

Goel, M., Sharma, P.K., Dureja, P., Rani, A., Uniyal, P.L. (2011). Antifungal activity of extracts of the lichens Parmelia reticulata, Ramalina roesleri, Usnea longissima and Stereocaulon himalayense. Archives of Phytopathology and Plant Protection 44(13): 1300-1311.

Hoskeri, J.H., Krishna, V., Amruthavalli, C. (2010). Effects of extracts from lichen Ramalina pacifica against clinically infectious bacteria. Researcher 2(3): 81-85.

Ingolfsdottir, K., Hjalmarsdottir, H.A., Sigurdsson, A., Gudjonsdottir, G.A., Brynjolfsdottir, A., Steingrimsson, O. (1997). In vitro susceptibility of Helicobacter pylori to protolichesterinic acid from the lichen Cetraria islandica. Antimicrobial agents and Chemotherapy 41(1): 215-217.

Javeria, S., Shahi, S.K., Shahi, M.P., Upreti, D.K. (2013). Parmotrema nilgherrense: potential antimicrobial activity against drug resistant pathogens. International Journal of Microbial Resource Technology 2(1): 36-40.

Kambar, Y., Vivek, M.N., Manasa, M., Kekuda, P.T.R., Onkarappa, R. (2014a). Antimicrobial activity of Ramalina conduplicans Vain. (Ramalinaceae). Science, Technology and Arts Research Journal 3(3): 57-62.

Kambar, Y., Vivek, M.N., Manasa, M., Vinayaka, K.S., Mallikarjun, N, Kekuda, P.T.R. (2014b). Antimicrobial activity of Leptogium burnetiae, Ramalina hossei, Roccella montagnei and Heterodermia diademata. International Journal of Pharmaceutical and Phytopharmacological Research 4(3): 164-168.

Karabulut, G., Ozturk, S. (2015). Antifungal activity of Evernia prunastri, Parmelia sulcata, Pseudevernia furfuracea var. furfuracea. Pakistan Journal of Botany 47(4): 1575-1579.

Karagöz, A., Doguöz, N., Zeybek, Z., Aslan, A. (2009). Antibacterial activity of some lichen extracts. Journal of Medicinal Plants Research 3(12): 1034-1039.

Karthikaidevi, G., Thirumaran, G., Manivannan, K., Anantharaman, P., Kathiresan, K., Balasubaramanian, T. (2009). Screening of the antibacterial properties of lichen Roccella belangeriana (Awasthi) from Pichavaram mangrove (Rhizophora sp.). Advances in Biological Research 3(3-4): 127-131.

Kekuda, P.T.R. (2014). Inhibitory activity of Usnea pictoides

G. Awasthi (Parmeliaceae) against urinary tract isolates.

Scholars Academic Journal of Biosciences 2(8): 561-563.

Kekuda, P.T.R., Raghavendra, H.L., Swathi, D., Venugopal, T.M., Vinayaka, K.S. (2012). Antifungal and cytotoxic

activity of Everniastrum cirrhatum (Fr.) Hale. Chiang Mai Journal of Science 39(1): 76-83.

Kekuda, P.T.R., Vivek, M.N., Kambar, Y., Manasa, M. (2014). Biocontrol potential of Parmotrema species against Colletotrichum capsici isolated from anthracnose of chilli. Journal of Biological and Scientific Opinion 2(2): 166-169.

Madamombe, I.T., Afolayan, A.J. (2003). Evaluation of antimicrobial activity of extracts from South African Usnea barbata. Pharmaceutical Biology 41(3): 199-202.

Nayaka, S., Upreti, D.K., Saxena, S. (2007). Muscicolous lichens of Indian subcontinent. In: Current Trends in Bryology. Nath, V., Asthana, A.K. (Editors). Bishen Singh Mahendra Pal Singh, Dehra Dun, India. pp 245-254.

Nayaka, S., Upreti, D.K., Phatak, S., Samuel, C. (2006). Preliminary observation of lichen flora of coconut and areca nut orchards of Goa, India. Phytotaxonomy 6:23- 25.

Park, I., Kim, J., Lee, Y., Shin, S. (2008). In vivo fungicidal activity of medicinal plant extracts against six phytopathogenic fungi. International Journal of Pest Management 54(1): 63-68.

Paudel, B., Bhattarai, H.D., Pandey, D.P., Hur, J.S., Hong, S.G., Kim, I., Yim, J.H. (2012). Antioxidant, antibacterial activity and brine shrimp toxicity test of some mountainous lichens from Nepal. Biological Research 45(4): 387-391.

Pavithra, G.M., Vinayaka, K.S., Rakesh, K.N., Junaid, S., Dileep, N., Kekuda, P.T.R, Siddiqua, S., Naik, A.S. (2013). Antimicrobial and antioxidant activities of a macrolichen Usnea pictoides G. Awasthi (Parmeliaceae). Journal of Applied Pharmaceutical Science 3(8): 154-160.

Rambo, T.R. (2010). Structure and composition of corticolous epiphyte communities in a Sierra Nevada old-growth mixed-conifer forest. The Bryologist 113(1): 55-71.

Rankovic, B., Mišic, M., Sukdolak, S. (2009). Antimicrobial activity of extracts of the lichens Cladonia furcata, Parmelia caperata, Parmelia pertusa, Hypogymnia physodes and Umbilicaria polyphylla. Biologia 64: 53-58.

Ranković, B., Ranković, D., Marić, D. (2010). Antioxidant and antimicrobial activity of some lichen species. Mikrobiologiia 79(6): 812-818.

Rout, J., Singha, B.A., Upreti, D.K. (2012). Lichen flora on betel nut (Areca catechu) palm tree from a pristine habitat in Southern Assam, India. Vegetos: An International Journal of Plant Research 25(1): 198-201.

Shivanna, R., Garampalli, R.H. (2015). Evaluation of fungistatic potential of lichen extracts against Fusarium solani (Mart.) Sacc. causing rhizome rot disease in Ginger. Journal of Applied Pharmaceutical Science 5 (10): 67-72.

Shukla, A.C., Chinlampianga, M., Verma, A., Dikshit, A., Upreti, D.K. (2011). Efficacy and potency of lichens of Mizoram as antimycotic agents. Indian Phytopathology 64 (4): 367-370.

Shukla, P., Upreti, D.K., Nayaka, S., Tiwari, P. (2014). Natural dyes from Himalayan lichens. Indian Journal of Traditional Knowledge 13(1): 195-201.

Swathi, D., Suchitha, Y., Kekuda, P.T.R., Venugopal, T.M., Vinayaka, K.S., Mallikarjun, N., Raghavendra, H.L. (2010). Antimicrobial, anthelmintic and insecticidal activity of a macrolichen Everniastrum cirrhatum (Fr.) Hale. International Journal of Drug Development and Research 2(4): 780-789.

Tiwari, P., Rai, H., Upreti, D.K., Trivedi, S., Shukla, P. (2011). Antifungal activity of a common Himalayan foliose lichen Parmotrema tinctorum ( Despr. ex Nyl.) Hale. Nature and Science 9(9): 167-171.

Turk, A.O., Yılmaz, M., Kıvan, M., Turk, H. (2003). The antimicrobial activity of extracts of the lichen Cetraria aculeata and its protolichesterinic acid constituent. Zeitschrift fur Naturforschung 58c: 850-854.

Turk, H., Yılmaz, M., Tay, T., Turk, A.O., Kıvanc, M. (2006). Antimicrobial activity of extracts of chemical races of the lichen Pseudevernia furfuracea and their physodic acid, chloroatranorin, atranorin, and olivetoric acid constituents. Zeitschrift fur Naturforschung 61c: 499-507.

Upreti, D.K., Bajpai, R., Nayaka, S. (2013). Ethnobotanically important Indian lichens. Ethnobotany 25: 33-40.

Upreti, D.K., Nayaka, S. (2008). Need for creation of lichen gardens and sanctuaries in India. Current Science 94(8): 976-978.

Verma, N., Behera, B.C., Parizadeh, H., Sharma, B.O. (2011). Bactericidal activity of some lichen secondary compounds of Cladonia ochrochlora, Parmotrema nilgherrensis and Parmotrema sancti-angelii. International Journal of Drug Development and Research 3(3):222- 232.

Verma, N., Behera, B.C., Sharma, B.O. (2012). Glucosidase inhibitory and radical scavenging properties of lichen metabolites salazinic acid, sekikaic acid and usnic acid. Hacettepe Journal of Biology and Chemistry 40 (1): 7-21.

Vinayaka, K.S., Kekuda, P.T.R., Nawaz, N.A.S., Junaid, S., Dileep, N., Rakesh, K.N. (2014). Inhibitory activity of Usnea pictoides G.Awasthi (Parmeliaceae) against Fusarium oxysporum f.sp. zingiberi and Pythium aphanidermatum isolated from rhizome rot of ginger. Life Sciences Leaflets 49: 17-22.

Vinayaka, K.S., Krishnamurthy, Y.L., Kekuda, P.T.R., Kumar, P.S.V., Sudharshan, S.J., Chinmaya, A. (2009). Larvicidal and wormicidal efficacy of methanolic extracts of five macrolichens collected from Bhadra wildlife sanctuary. Biomedicine 29(4): 327-331.

Vivek, M.N., Kambar, Y., Manasa, M., Kekuda, P.T.R., Vinayaka, K.S. (2014a). Radical scavenging and antibacterial activity of three Parmotrema species from Western Ghats of Karnataka, India. Journal of Applied Pharmaceutical Science 4(3): 86-91.

Vivek, M.N., Manasa, M., Kambar, Y., Kekuda, P.T.R., Raghavendra, H.L. (2014b). Antifungal efficacy of three bioactive Parmotrema species from Western Ghats of Karnataka, India. International Journal of Agriculture and Crop Sciences 7(12): 968-973.

Walker, F.J., James, P.W. (1980). A revised guide to microchemical techniques for the identification of lichen substances. Bulletin of British Lichenological Society 46 (Supp l): 13-29.

Wei, X., Jeon, H., Han, K.S., Koh, Y.J., Hur, J. (2008).

Antifungal activity of lichen-forming fungi against Colletotrichum acutatum on hot pepper. The Plant Pathology Journal 24(2): 202-206.

Yilmaz, M., Turk, A.O., Tay, T., Kıvanc, M. (2004). The antimicrobial activity of extracts of the lichen Cladonia foliacea and its (-)-usnic acid, atranorin, and fumarprotocetraric acid constituents. Zeitschrift fur Naturforschung 59c: 249-254.

Zambare, V.P., Christopher, L.P. (2012). Biopharmaceutical potential of lichens. Pharmaceutical Biology 50(6):778-98.

Downloads

Published

28.09.2015

How to Cite

Kekuda T.R, P., M.C, R., Firdose, G., P, V., & K.S, V. (2015). Antimicrobial Activity of Selected Corticolous Macrolichens . Journal of Science, Technology and Arts Research, 4(3), 169–174. Retrieved from https://journals.wgu.edu.et/index.php/star/article/view/261

Issue

Section

Original Research

Categories

Plaudit

Most read articles by the same author(s)

1 2 > >>