Isolation and Screening of Industrially Important Fungi from the Soils of Western Ghats of Agumbe and Koppa, Karnataka, India
Keywords:
Western Ghats, Soil, Agumbe, Hydrolytic EnzymesAbstract
The present study is an attempt to isolate the fungi from the unexplored soils of Western Ghats and screen them for t e production of some very important hydrolytic enzymes such as amylase, cellulose, CMCase, protease, lipase and pigment production. In this work, more than 200 fungal isolates were isolated from the forest soil were screened for the production of extracellular enzymes, identification of the screened isolates and secondary screening for amylase production was done. A total of 167 isolates were characterized, in them some up to species level. Aspergillus, Penicillium, Trichoderma and Cladosporium were predominated. A total of 14 isolates were selected for the secondary screening for amylase production. Aspergillus isolate 199 and Aspergillus isolate 43 produced more protein (169±07 and 160±04µg) respectively. Amylase assay also revealed greater activities (4.98±0.06 and 4.93± 0.07µ moles/ ml) in Aspergillus isolate 199 and Aspergillus isolate 43 respectively.
Downloads
Metrics
References
Aneja, K.R. (1996). Experiments in Microbiology, Plant Pathology, Tissue Culture and Mushroom Cultivation. 2nd Ed. Wishwa Prakashan, New Delhi, Pp. 451.
Arunsasi., Kani, M., Panneerselvam, A., Jegadeesh, G., Muthu, K and Kumar, M.R. (2010). Optimizing the conditions of α- amylase by an Estuarine strain of Aspergillus spp. African Journal of Microbiology Research 4 (8): 581-586.
Bakri, Y., Mangali, M and Thonart, P. (2009). Isolation and Identification of a New Fungal Strain for Amylase Biosynthesis. Polish Journal of Microbiology 58(3):269-273.
Banakar, S.P., Thippeswamy, B., Thirumalesh, B.V and Naveenkumar, K.J. (2012). Isolation, production and partial purification of fungal amylase from forest soils of Bhadra Wildlife Sanctury, Western Ghats. Inventi Rapid: Pharmacy, Biotechnology and Microbiology 3:1-7.
Barnett, H.L and Hunter, B.B. (1992). Illustrated genera of imperfect fungi. 3rd Edition. Burgess publishing company, Minnessota. Pp-241.
Buzzini, P and Martini, A. (2002). Extracellular enzymatic activity profiles in yeast and cassava waste dumpsite in Minna, Niger State, Nigeria. African Journal of Microbiology Research 3 (4): 143-146.
Damisa, D., Ameh, J.B and Egbe, N.E.L. (2011). Cellulase Production by native Aspergillus niger obtained from soil environments. Fermentation Technology and Bioengineering 1: 62-70.
Gautam, S.P., Bundela, P.S., Pandey. A.K., Awasthi,
M.K and Sarsaiya, S. (2010). Screening of cellulolytic fungi for management of municipal solid waste. Journal of Applied Sciences in Environmental Sanitation 5(4): 391-395.
Gupta, R., Gigras, P., Mohapatra, H., Goswamy, V.K and Chauhan, B. (2003). Microbial α-amylases: a biotechnological perspective. Process Biochemistry 04:1-18.
Haki, G.D and Rakshith, S.K. (2003). Developments in industrially important thermostable enzymes; a review. Bioresource Technology. 89: 17-34.
Kathiresan, K and Manivannan, S. (2006). Alpha amylase production by Penicillium fellutanun isolated from Mangroove rhizosphere soil. African Journal of Biotechnology 3(10): 829-832.
Kirk, O., Borchert, T.V and Fuglsang, C.C. (2002). Industrial enzyme applications. Current Opinion in Biotechnology 13: 345–351.
Kundu, A.K and Das, S. (1970). Production of Amylase in Liquid Culture by a Strain of Aspergillus oryzae, Applied Microbiology 19: 598-603.
Lowry, O.H., Rosebrough, N.J., Farr, A.L and Randall,
R.J. (1951). Protein measurement with the folin phenol regent. Journal of General Microbiology 131: 3017-3027.
Malloch, D. (1981). Moulds, their Isolation, Cultivation and Identification. University of Toronto Press, Toronto. Pp- 97.
McGinnis, M.R., D’Amato, R.F and Land, G.A. (1982). Pictorial Handbook of Medically Important Fungi and Aerobic Actinomycetes. Praeger Publishers, New York. Pp- 160.
Miller, G.L. (1959). Use of Dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31: 426-248.
Mishra, B.K and Dadhich, S.K. (2010). Production of amylase and xylanase enzymes from soil fungi of Rajasthan. Journal of Advances in developmental Research 1(1): 21-23.
Moallaei, H., Zaini, F., Pihet, M., Mahmoudi, M and Hashemi, J. (2006). Isolation of Keratinophilic Fungi from Soil Samples of Forests and Farm Yards. Iranian Journal of Public Health 35(4): 62-69.
Oyeleke, S. B and Oduwole, A.A. (2009). Production of amylase by bacteria isolated from a cassava waste dumpsite in Minna, Niger state, Nigeria. African Journal of Microbiology Research 3(4): 143-146.
Pothiraj, C., Balaji, P and Eyini, M. (2006). Enhanced production of cellulases by various fungal cultures in solid state fermentation of cassava waste. African Journal of Biotechnology 5(20): 1882-1885.
Rajan, A., Kumar, D.R.S and Nair, A.J. (2011). Isolation of a novel alkaline lipase producing fungus Aspergillus fumigatus MTCC 9657 from aged and crude rice bran oiland quantification by HPTLC. International Journal of Biological Chemistry 5(2): 116-126.
Samira, M., Mohammad, R and Gholamreza, G. (2011). Carboxymethyl cellulose and filter paperase activity of new strains isolated from Persian Gulf. Microbiology Journal 1(1): 8-16.
Sathyaprabha, G., Panneerselvam, A and Muthukkumarasamy, S. (2011). Production of Cellulase and Amylase from wild and mutated fungal isolates. E-Journal of Life Sciences 1(1): 39- 45.
Srinivasan, M.C. (2004). Practical Mycology for Industrial Biotechnologists. Tata McGraw-Hill Publishing Company Limited, New Delhi, Pp-243.
Tiwari, K.L., Jadhav, S.K and Fatima, A. (2007). Culture condition for the production of thermostable amylase by Penicillium rugulosum. Global Journal of Biotechnology and Biochemistry 2(1): 21-24.
Downloads
Published
How to Cite
License
Copyright (c) 2012 journal of Science, Technology and Arts Research
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
STAR © 2023 Copyright; All rights reserved