Antimicrobial Activity of Ramalina conduplicans Vain. (Ramalinaceae)
Keywords:
Ramalina conduplicans, Antimicrobial, Agar well diffusion, Poisoned food techniqueAbstract
The members of the genus Ramalina (Ramalinaceae) are fruticose lichens growing on various types of substrata. The present study was conducted with an aim of determining antimicrobial activity of Ramalina conduplicans Vain. The powdered lichen material was extracted sequentially using petroleum ether, ethyl acetate and ethanol. The solvent extracts were screened for antibacterial activity by Agar well diffusion assay against 15 bacteria which included reference strains and isolates from burn, dental caries and urinary tract infections. Poisoned food technique was performed to determine antifungal effect of solvent extracts against fungal isolates from anthracnose of chilli, foot rot of finger millet and mouldy grains of sorghum. Usnic acid, Salazinic acid and Sekikaic acid were detected in the lichen. The solvent extracts were shown to exhibit dose dependent inhibitory activity against test bacteria. Ethyl acetate extract inhibited reference bacterial strains to higher extent. Among solvent extracts, only ethanol extract inhibited all urinary tract bacteria. S. aureus isolates from burn were inhibited to higher extent when compared to S. mutans isolates from dental caries. The solvent extracts exhibited varied inhibitory activity against test fungi. Ethyl acetate extract inhibited Alternaria sp., Aspergillus flavus and Sclerotium rolfsii to higher extent while Colletotrichum capsici and Helminthosporium sp. were inhibited to higher extent by petroleum ether and ethanol extract respectively. The observed inhibitory potential of solvent extracts of R. conduplicans could be ascribed to the presence of secondary metabolites. The lichen can be used in the treatment of bacterial infections and to manage plant pathogenic fungi.
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Agboke, A.A. and Esimone, C.O. (2011). Antimicrobial evaluation of the interaction between methanol extract of the lichen, Ramalina farinacea (Ramalinacea) and Ampicilin against clinical isolates of Staphylococcus aureus. Journal of Medicinal Plants Research 5(4): 644- 648.
Awasthi, D.D. (2000). A compendium of the macrolichens from India, Nepal and Sri Lanka. Bishen Singh Mahendra Pal Singh, Dehradun, India.
Bhattarai, T., Subba, D. and Subba, R. (1999). Nutritional value of some edible lichens of east Nepal. Journal of Applied Botany and Food Quality – Angewandte Botanik 73:11-18.
Culberson, C.F. and Kristinsson, H. (1970). A standardized method for the identification of lichen products. Journal of Chromatography 46: 85-93.
Culberson, C.F. (1972). Improved conditions and new data for the identification of lichen products by a standardized thin layer chromatographic method. Journal of Chromatography 72: 113-125.
Dellavalle, P.D., Cabrera, A., Alem, D., Larranaga, P. and Ferreira, F., Rizza, M.D. (2011). Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chilean Journal of Agricultural Research 71(2): 231-239.
Dileep, N., Junaid, S., Rakesh, K.N., Kekuda, P.T.R. and Nawaz, A.S.N. (2013). Antifungal activity of leaf and pericarp extract of Polyalthia longifolia against pathogens causing rhizome rot of ginger. Science, Technology and Arts Research Journal 2(1): 56-59.
Fu, W., Zhao, Z., Guo, S. and Guo, S. (2008). The lichen genus Ramalina from northeastern China. Mycosystema 27(1): 91-98.
Goel, M., Sharma, P.K., Dureja, P., Rani, A. and Uniyal, P.L. (2011). Antifungal activity of extracts of the lichens Parmelia reticulata, Ramalina roesleri, Usnea longissima and Stereocaulon himalayense. Archives of Phytopathology and Plant Protection 44(13): 1300-1311.
Hoskeri, J.H., Krishna, V. and Amruthavalli, C. (2010). Effects of extracts from lichen Ramalina pacifica against clinically infectious bacteria. Researcher 2(3): 81-85.
Javeria, S., Shahi, S.K., Shahi, M.P. and Upreti, D.K. (2013). Parmotrema nilgherrense: potential antimicrobial activity against drug resistant pathogens. International Journal of Microbial Resource Technology 2(1): 36-40.
Karagöz, A., Dogruöz, N., Zeybek, Z. and Aslan, A. (2009). Antibacterial activity of some lichen extracts. Journal of Medicinal Plants Research 3(12): 1034-1039.
Kekuda, P.T.R., Junaid, S., Dileep, N., Rakesh, K.N. and Vinayaka, K.S. (2013). Anticaries activity of Usnea pictoides G. Awasthi -A macrolichen from Western Ghats of Karnataka, India. Science, Technology and Arts Research Journal 2(4): 87-90.
Kekuda, P.T.R., Vinayaka, K.S., Kumar, P.S.V. and Sudharshan, S.J. (2009). Antioxidant and antibacterial activity of lichen extracts, honey and their combination. Journal of Pharmacy Research 2(12): 1875-1878.
Kosanić, M., Šeklić, D., Marković, S. and Ranković, B. (2014). Evaluation of antioxidant, antimicrobial and anticancer properties of selected lichens from Serbia. Digest Journal of Nanomaterials and Biostructures 9(1): 273-287.
Kumar, A.H.S., Kekuda, P.T.R., VInayaka, K.S., Swathi, D. and Venugopal, T.M. (2011). Anti-obesity (Pancreatic lipase inhibitory) activity of Everniastrum cirrhatum (Fr.) Hale (Parmeliaceae). Pharmacognosy Journal 3(19): 65-
Kumar, P.S.V., Kekuda, P.T.R., Vinayaka, K.S., Swathi, D. and Chinmaya, A. (2010a). Insecticidal efficacy of Ramalina hossei H. Magn and G. Awasthi and Ramalina conduplicans Vain- Macrolichens from Bhadra wildlife sanctuary, Karnataka. Biomedicine 30(1): 100-102.
Kumar, P.S.V., Kekuda, P.T.R., Vinayaka, K.S., Swathi, D., Mallikarjun, N. and Nishanth, B.C. (2010b). Studies on proximate composition, antifungal and anthelmintic activity of a macrolichen Ramalina hossei H. Magn and G. Awasthi. International Journal of Biotechnology and Biochemistry 6(2): 191-201.
Lin, C. (2009). A preliminary study of the lichen genus Ramalina at Mt. Yangtou, Hualien County, Eastern Taiwan. Collection and Research 22: 131-134.
Luo, H., Wei, X., Yamamoto, Y., Liu, Y., Wang, L., Jung, J.S., Koh, Y.J. and Hur, J. (2010). Antioxidant activities of edible lichen Ramalina conduplicans and its free radical- scavenging constituents. Mycoscience 51(5): 391-395.
Panea, C., Piccolo, A., Spaccini, R., Celanoc, G., Villeccoa,
D. and Zaccardellia, M. (2013). Agricultural waste-based composts exhibiting suppressivity to diseases caused by the phytopathogenic soil-borne fungi Rhizoctonia solani and Sclerotinia minor. Applied Soil Ecology 65: 43-51.
Park, I., Kim, J., Lee, Y. and Shin, S. (2008). In vivo fungicidal activity of medicinal plant extracts against six phytopathogenic fungi. International Journal of Pest Management 54(1): 63-68.
Pavithra, G.M., Vinayaka, K.S., Rakesh, K.N., Junaid, S., Dileep, N., Kekuda, P.T.R., Siddiqua, S. and Naik, A.S. (2013). Antimicrobial and antioxidant activities of a macrolichen Usnea pictoides G. Awasthi (Parmeliaceae). Journal of Applied Pharmaceutical Science 3(8): 154-160.
Perry, N.B., Benn, M.H., Brennan, N.J., Burgess, E.J., Ellis, G., Galloway, D.J., Lorimer, S.D. and Tangney, R.S. (1999). Antimicrobial, antiviral and cytotoxic activity of New Zealand lichens. Lichenologist 31(6): 627-636.
Santiago, K.A.A., Borricano, J.N.C., Canal, J.N., Marcelo, D.M.A., Perez, M.C.P. and dela Cruz, T.E.E. (2010). Antibacterial activities of fruticose lichens collected from selected sites in Luzon Island, Philippines. Philippine Science Letters 3(2): 18-29.
Shrestha, G. and St. Clair, L.L. (2013). Antimicrobial activity of extracts from two lichens Ramalina menziesii and Usnea lapponica. Bulletin of the California Lichen Society 20(1): 5-10.
Shukla, A.C., Chinlampianga, M., Verma, A., Dikshit, A. and Upreti, D.K. (2011). Efficacy and potency of lichens of Mizoram as antimycotic agents. Indian Phytopathology 64 (4): 367-370.
Shukla, P., Upreti, D.K. and Tiwari, P. (2014). Assessment of dye yielding potential of Indian lichens. Indian Journal of Plant Sciences 3(1): 57-63.
Smith, R.D. and Coast, J. (2002). Antimicrobial resistance: a global response. Bulletin of the World Health Organization 80(2): 126-133.
Ul Haq, M., Reshi, Z.A., Upreti, D.K. and Sheikh, M.A. (2012). Lichen wealth of Jammu and Kashmir- A promising plant source for bioprospection. Life Science Journal 9(4): 926- 929.
Upreti, D.K., Divakar, P.K. and Nayaka, S. (2005). Commercial and ethnic use of lichens in India. Economic Botany 59: 269-273.
Verma, N., Behera, B.C., Parizadeh, H. and Sharma, B.O. (2011). Bactericidal activity of some lichen secondary compounds of Cladonia ochrochlora, Parmotrema nilgherrensis and Parmotrema sancti-angelii. International Journal of Drug Development & Research 3(3): 222-232.
Vinayaka, K.S., Karthik, S., Nandini, K.C. and Kekuda, P.T.R. (2013). Amylase inhibitory activity of some macrolichens of Western Ghats, Karnataka, India. Indian Journal of Novel Drug Delivery 5(4): 225-228.
Vinayaka, K.S., Kekuda, P.T.R., Nawaz, N.A.S., Junaid, S., Dileep, N. and Rakesh, K.N. (2014). Inhibitory activity of Usnea pictoides G.Awasthi (Parmeliaceae) against Fusarium oxysporum f.sp. zingiberi and Pythium aphanidermatum isolated from rhizome rot of ginger. Life Sciences Leaflets 49: 17-22.
Vinayaka, K.S., Kumar, P.S.V., Kekuda, P.T.R., Krishnamurthy, Y.L., Mallikarjun, N. and Swathi, D. (2009). Proximate composition, antioxidant, anthelmintic and insecticidal activity of a macrolichen Ramalina conduplicans Vain. (Ramalinaceae). European Journal of Applied Sciences 1(3): 40-46.
Vivek, M.N., Kambar, Y., Manasa, M., Kekuda, P.T.R. and Vinayaka, K.S. (2014). Radical scavenging and antibacterial activity of three Parmotrema species from Western Ghats of Karnataka, India. Journal of Applied Pharmaceutical Science 4(3): 86-91.
Wang, L.S., Narui, T., Harada, H., Culberson, C.F. and Culberson, W.L. (2001). Ethnic uses of lichens in Yunnan, China. Bryologist 104: 345-349.
Wei, X., Jeon, H., Han, K.S., Koh, Y.J. and Hur, J. (2008).
Antifungal activity of lichen-forming fungi against Colletotrichum acutatum on hot pepper. The Plant Pathology Journal 24(2): 202-206.
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