Characterization and Antibacterial Activity of a Glycoside Antibiotic from Streptomyces variabilis PO-178

Authors

  • Prashith Kekuda T.R Kuvempu University
  • Onkarappa R Kuvempu University
  • Jayanna N.D Kuvempu University

Keywords:

Streptomyces variabilis PO-178, Agumbe, Glycoside, Antibacterial activity, Spectral analysis

Abstract

In the present study, characterization and antibacterial activity of a glycoside antibiotic from fermentation broth of bioactive Streptomyces variabilis PO-178 recovered previously from Western Ghat soil of Agumbe, Karnataka, India was investigated. Mass cultivation of the strain PO-178 was carried out in Starch casein nitrate broth. The fermentation broth was extracted using butanol solvent in a separation funnel. The concentrated butanol extract was subjected to chromatographic techniques viz., thin layer and silica gel column chromatography for purification of bioactive components. Out of 3 fractions (A, B and C) obtained, only one fraction (C) displayed inhibitory activity against Staphylococcus aureus and Pseudomonas aeruginosa. The bioactive fraction C was subjected to spectral analysis (LC-MS, IR, 1H-NMR and 13C-NMR) in order to determine the structure. On the basis result of spectral analysis, the purified fraction was predicted as a glycoside antibiotic with molecular weight 514 and molecular formula C24H34O12. The antibiotic displayed marked inhibitory activity against S. aureus as revealed by wider zones of inhibition and low minimum inhibitory concentration value.

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Author Biographies

Prashith Kekuda T.R, Kuvempu University

 

 

Onkarappa R, Kuvempu University

 

 

Jayanna N.D, Kuvempu University

 

 

References

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Published

28.12.2014

How to Cite

Kekuda T.R, P., Onkarappa R, & Jayanna N.D. (2014). Characterization and Antibacterial Activity of a Glycoside Antibiotic from Streptomyces variabilis PO-178. Journal of Science, Technology and Arts Research, 3(4), 116–121. Retrieved from https://journals.wgu.edu.et/index.php/star/article/view/526

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Section

Original Research

Categories

Plaudit

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