Antibacterial, Cytotoxic and Antioxidant Potential of Vitex Negundo Var. Negundo and Vitex Negundo Var. Purpurascens

A Comparative Study

Authors

  • Prashith Kekuda TR SRNMN College of Applied Sciences
  • Manasa M SRNMN College of Applied Sciences
  • Poornima G SRNMN College of Applied Sciences
  • Abhipsa V SRNMN College of Applied Sciences
  • Rekha C SRNMN College of Applied Sciences
  • Shivaleela P. Upashe Wallaga University
  • Raghavendra HL Wallaga University

Keywords:

Vitex negundo, Agar well diffusion, DPPH, Ferric reducing, Total phenolic, Brine shrimp

Abstract

The present study was carried out to estimate the content of total phenolics, flavonoids, negundoside and agnuside and to determine antibacterial, cytotoxic and antioxidant efficacy of methanol extract of two varieties of Vitex negundo namely V. negundo var. negundo (Vnvn) and V. negundo var. purpurascens (Vnvp). Total phenolic and flavonoid content of leaf extracts were estimated by Folin-Ciocalteau reagent and Aluminium chloride colorimetric estimation method respectively. Negundoside and agnuside content were estimated using HPLC. Antibacterial activity of both the extracts was tested by Agar well diffusion assay. Cytotoxicity of extract was evaluated by Brine shrimp lethality bioassay. Antioxidant activity of extracts was determined by DPPH free radical scavenging and Ferric reducing assay. The content of total phenolics and flavonoid were higher in extract of Vnvp. Negundoside and agnuside contents were high in Vnvn and Vnvp respectively. Leaf extract of Vnvp was shown to display marked antibacterial, cytotoxic and antioxidant activity when compared with extract of Vnvn. Both the varieties of V. negundo showed marked antibacterial, cytotoxic and antioxidant activity. The extract of Vnvp displayed marked bioactivities than that of extract of Vnvn. The presence of high phenolic and flavonoid content could be attributed to the marked bioactivities of Vnvp.

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Author Biographies

Prashith Kekuda TR, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road,
Shivamogga-577201,
Karnataka, India

Manasa M, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road,
Shivamogga-577201,
Karnataka, India

Poornima G, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road,
Shivamogga-577201,
Karnataka, India

Abhipsa V, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road,
Shivamogga-577201,
Karnataka, India

Rekha C, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S Campus, Balraj Urs Road,
Shivamogga-577201,
Karnataka, India

Shivaleela P. Upashe, Wallaga University

College of Medical and Health Sciences, Wollega University, Post Box No: 395, Nekemte, Ethiopia

Raghavendra HL, Wallaga University

College of Medical and Health Sciences, Wollega University, Post Box No: 395, Nekemte, Ethiopia

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Published

30.09.2013

How to Cite

Kekuda TR, P., M, M., G, P., V, A., C, R., P. , S., & HL, R. (2013). Antibacterial, Cytotoxic and Antioxidant Potential of Vitex Negundo Var. Negundo and Vitex Negundo Var. Purpurascens: A Comparative Study. Journal of Science, Technology and Arts Research, 2(3), 59–68. Retrieved from https://journals.wgu.edu.et/index.php/star/article/view/525

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