Antimicrobial and Antioxidant activity of Anaphalislawii (Hook.f.) Gamble

Authors

  • Prashith Kekuda T.R SRNMN College of Applied Sciences
  • Rakesh K.N SRNMN College of Applied Sciences
  • Dileep N SRNMN College of Applied Sciences
  • Syed Junaid SRNMN College of Applied Sciences
  • Pavithra G.M SRNMN College of Applied Sciences
  • Soumya S. Gunaga SRNMN College of Applied Sciences
  • Megha V.H SRNMN College of Applied Sciences
  • Raghavendra H.L Wallaga University

Keywords:

Anaphalislawii, Agar well, diffusion, Antioxidant, activity, Flavonoid, Total phenolic

Abstract

The present study was undertaken to investigate antimicrobial and antioxidant activities of leaf and flower extracts of Anaphalislawii (Hook.f.) Gamble (Compositae). The powdered leaves and flowers were extracted with methanol in soxhlet extraction assembly. The antimicrobial activity of leaf extract (LE) and flower extract (FE) were determined against a panel of 9 bacteria and 2 fungi by Agar well diffusion method. Antioxidant activity of LE and FE was screened by DPPH free radical scavenging, Ferric reducing and Metal chelating activity. Total phenolic content and flavonoid content of extracts was determined by Folin-Ciocalteu method and Aluminium chloride colorimetric estimation method respectively. Both LE and FE caused inhibition of test bacteria and fungi in a concentration dependent manner. Gram positive bacteria were more susceptible to LE and FE when compared to Gram negative bacteria. On comparing with Candida albicans, susceptibility to extracts were found higher in Cryptococcus neoformans. The LE and FE dose dependently scavenged DPPH free radical with IC50 value of 6.01 and 5.20µg/ml respectively. An increase in the absorbance of reaction mixture with increase in concentrations of extracts showed reducing potential of LE and FE in ferric reducing assay. In metal chelating assay, both LE and FE caused chelation of ferrous ion with IC50 value of 180.98 and 152.89µg/ml respectively. The phytochemical analysis revealed the presence of flavonoids, saponins and tannins in both the extracts. Total phenolic and flavonoid contents were high in FE than LE. Overall, FE showed potent antioxidant and antimicrobial activity when compared with LE and it could be related to the high phenolic and flavonoid content in FE. Further studies on isolation of active principles from extracts and testing their bioactivities are to be carried out.

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Author Biographies

Prashith Kekuda T.R, SRNMN College of Applied Sciences

Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, BalrajUrs Road, Shivamogga-577201, Karnataka, India

Rakesh K.N , SRNMN College of Applied Sciences

Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, BalrajUrs Road, Shivamogga-577201, Karnataka, India

Dileep N, SRNMN College of Applied Sciences

Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, BalrajUrs Road, Shivamogga-577201, Karnataka, India

Syed Junaid, SRNMN College of Applied Sciences

Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, BalrajUrs Road, Shivamogga-577201, Karnataka, India

Pavithra G.M, SRNMN College of Applied Sciences

Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, BalrajUrs Road, Shivamogga-577201, Karnataka, India

Soumya S. Gunaga, SRNMN College of Applied Sciences

Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, BalrajUrs Road, Shivamogga-577201, Karnataka, India

Megha V.H, SRNMN College of Applied Sciences

Department of Microbiology, SRNMN College of Applied Sciences, NES Campus, BalrajUrs Road, Shivamogga-577201, Karnataka, India

Raghavendra H.L, Wallaga University

College of Medical and Health Sciences, Wollega University, Post Box No: 395, Nekemte, Ethiopia

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Published

30.09.2012

How to Cite

Kekuda T.R, P., K.N , R., n, D., Junaid, S., G.M, P., S. Gunaga, S., V.H, M., & H.L, R. (2012). Antimicrobial and Antioxidant activity of Anaphalislawii (Hook.f.) Gamble. Journal of Science, Technology and Arts Research, 1(3), 08–16. Retrieved from https://journals.wgu.edu.et/index.php/star/article/view/25

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