Antiradical and Antimicrobial Activity of Atylosia lineata Wt. and Arn.

Authors

  • Prashith Kekuda T.R SRNMN College of Applied Sciences
  • Akarsh S SRNMN College of Applied Sciences
  • Darshini S.M SRNMN College of Applied Sciences
  • Prafulla D SRNMN College of Applied Sciences
  • Raghavendra H.L SRNMN College of Applied Sciences

Keywords:

Atylosia lineate, Agar well diffusion, Poisoned food technique, DPPH, ABTS, Total phenolic

Abstract

The present study was conducted to determine antimicrobial and radical scavenging activity of Atylosia lineata Wt. & Arn. (Fabaceae). The shade dried and powdered leaves were extracted using methanol. Antibacterial and antifungal activity of extract was determined by agar well diffusion and poisoned food technique respectively. Antiradical efficacy was determined by DPPH and ABTS radical scavenging assays. Total phenolic content of leaf extract was estimated by Folin-Ciocalteau Reagent method. The extract was effective against all test bacteria with marked inhibitory effect against Pseudomonas aeruginosa. Gram negative bacteria were susceptible to higher extent than Gram positive bacteria. The extract inhibited the growth of all test fungi to >50%. Among fungi, Colletotrichum capsici was inhibited to higher extent. The extract scavenged DPPH and ABTS radicals in dose dependent manner. At extract concentration 100µg/ml and higher, >90% scavenging of both radicals was observed. The total phenolic content of leaf extract was found to be 81.36mg GAE/g of extract. The plant appears promising to treat infectious diseases and oxidative damage.

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Author Biographies

Prashith Kekuda T.R, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S campus, Balraj Urs Road,
Shivamogha-577201, Karnataka

Department of Microbiology, Sahyadri Science College (Autonomous), Shivamogga-577203, Karnataka

Akarsh S, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S campus, Balraj Urs Road,
Shivamogha-577201, Karnataka

Darshini S.M, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S campus, Balraj Urs Road,
Shivamogha-577201, Karnataka

Prafulla D, SRNMN College of Applied Sciences

Department of Microbiology, S.R.N.M.N College of Applied Sciences, N.E.S campus, Balraj Urs Road,
Shivamogha-577201, Karnataka

Raghavendra H.L, SRNMN College of Applied Sciences

College of Medical and Health Sciences, Wollega University, Post Box No: 395, Nekemte, Ethiopia

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Published

30.09.2015

How to Cite

Kekuda T.R, P., S, A., S.M, D., D, P., & H.L, R. (2015). Antiradical and Antimicrobial Activity of Atylosia lineata Wt. and Arn. Journal of Science, Technology and Arts Research, 4(3), 180–183. Retrieved from https://journals.wgu.edu.et/index.php/star/article/view/263

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Section

Original Research

Categories

Plaudit

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